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Reproducible In Vitro Tissue Culture Model to Study Basic Mechanisms of Calcific Aortic Valve Disease: Comparative Analysis to Valvular Interstitials Cells

Reproducible In Vitro Tissue Culture Model to Study Basic Mechanisms of Calcific Aortic Valve Disease: Comparative Analysis to Valvular Interstitials Cells

Posted on August 25, 2021July 25, 2021 by Jack

The hallmarks of calcific aortic valve illness (CAVD), an lively and controlled course of involving the creation of calcium nodules, lipoprotein accumulation, and power irritation, are the numerous modifications that happen within the composition, group, and mechanical properties of the extracellular matrix (ECM) of the aortic valve (AV).

Most analysis concerning CAVD is predicated on experiments utilizing two-dimensional (2D) cell tradition or artificially created three-dimensional (3D) environments of valvular interstitial cells (VICs). Because the valvular ECM has a strong affect in regulating pathological occasions, we developed an in vitro AV tissue tradition mannequin, which is extra intently ready to mimic pure circumstances to research mobile responses underlying CAVD.

AV leaflets, remoted from the hearts of 6-8-month-old sheep, had been mounted with needles on silicon rubber rings to obtain passive rigidity and handled in vitro below pro-degenerative and pro-calcifying circumstances. The degeneration of AV leaflets progressed over time, commencing with the primary seen calcified domains after 14 d and winding up with the distinct formation of calcium nodules, heightened stiffness, and clear disruption of the ECM after 56 d.

Both the expression of pro-degenerative genes and the myofibroblastic differentiation of VICs had been altered in AV leaflets in contrast to that in VIC cultures. In this research, we’ve got established an simply relevant, reproducible, and cost-effective in vitro AV tissue tradition mannequin to research pathological mechanisms underlying CAVD.

The valvular ECM and life like VIC-VEC interactions mimic pure circumstances extra intently than VIC cultures or 3D environments. The utility of varied tradition circumstances allows the examination of totally different pathological mechanisms underlying CAVD and may lead to a greater understanding of the molecular mechanisms that lead to VIC degeneration and AS.

Our mannequin gives a helpful software to research the complicated pathobiology of CAVD and can be utilized to determine potential therapeutic targets for slowing illness development.

Establishment of hematopoietic tissue main cell cultures from the enormous freshwater prawn Macrobrachium rosenbergii

The big freshwater prawn Macrobrachium rosenbergii is one of an important aquaculture species in Southeast Asia. In this research, in vitro tradition of its hematopoietic tissue cells was achieved and characterised to be used as a software to research its pathogens that trigger main farm losses.

By transmission electron microscopy, the ultrastructure of the first tradition cells was related to that of cells lining intact hematopoietic tissue lobes. Proliferating cell nuclear antigen (PCNA) (a marker for hematopoietic stem cell proliferation) was detected in some of the aesthetic cells by polymerase chain response (PCR) testing and movement cytometry.

Using a particular staining methodology to detect phenoloxidase exercise and utilizing PCR to detect expression markers for semigranular and granular hemocytes (e.g., prophenoloxidase activating enzyme and prophenoloxidase) revealed that some of the first cells had been ready to differentiate into mature hemocytes inside 24 h.

These outcomes confirmed that some cells within the cultures had been hematopoietic stem cells that could possibly be used to research different fascinating analysis matters (e.g. host pathogen interactions and improvement of an immortal hematopoietic stem cell line).

Towards Self-Regulated Bioprocessing: A Compact Benchtop Bioreactor System for Monitored and Controlled 3D Cell and Tissue Culture.

Bioreactors are essential instruments for the manufacturing of residing cell-based tissue engineered merchandise. However, to attain the market efficiently, greater levels of automation, in addition to a decreased footprint nonetheless want to be reached. In this research, the use of a benchtop bioreactor for in vitro perfusion tradition of scaffold-based tissue engineering constructs is assessed. A low-footprint benchtop bioreactor system is designed, comprising a single-use fluidic elements and a bioreactor housing.

The bioreactor is operated utilizing an in-house developed program and the tradition surroundings is monitored by particularly designed sensor ports. A gas-exchange module is integrated permitting for warmth and mass transfers. Titanium-based scaffolds are seeded with human periosteum-derived cells and cultured up to three weeks. The benchtop bioreactor constructs are in contrast to benchmark perfusion programs.

Live/Dead stainings, DNA quantifications, glucose consumption, and lactate manufacturing assays affirm that the constructs cultured within the benchtop bioreactor grew equally to the benchmark programs. Manual regulation of the system set factors enabled environment friendly alteration of the tradition surroundings in phrases of temperature, pH, and dissolved oxygen.

This research gives the mandatory foundation for the event of low-footprint, automated, benchtop perfusion bioreactors and allows the implementation of lively surroundings management.

In vitro transplantation of spermatogonial stem cells remoted from human frozen-thawed testis tissue can induce spermatogenesis below three-dimensional tissue tradition circumstances.

Sperm manufacturing is one of probably the most complicated organic processes within the physique. In vitro manufacturing of sperm is one of an important targets of researches within the area of male infertility remedy, which is essential in male most cancers sufferers handled with gonadotoxic strategies and medicines. In this research, we study the development of spermatogenesis after transplantation of spermatogonial stem cells below circumstances of testicular tissue tradition.

Testicular tissue samples from azoospermic sufferers had been obtained after which these had been freeze-thawed. Spermatogonial stem cells had been remoted by two enzymatic digestion steps and the identification of these cells was confirmed by detecting the PLZF protein.

These cells, after being labeled with DiI, had been transplanted in azoospermia grownup mice mannequin. The host testes had been positioned on agarose gel as tissue tradition system. After Eight weeks, histomorphometric, immunohistochemical and molecular research had been carried out.

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

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Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

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Description: A sandwich quantitative ELISA assay kit for detection of Human Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

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Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

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Description: Human

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Description: Human Connective tissue growth factor (CTGF) ELISA Kit employs a two-site sandwich ELISA to quantitate CTGF in samples.

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Description: Human Connective tissue growth factor (CTGF) ELISA Kit employs a two-site sandwich ELISA to quantitate CTGF in samples.

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Description: Human Connective tissue growth factor (CTGF) ELISA Kit employs a two-site sandwich ELISA to quantitate CTGF in samples.

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Human Connective Tissue Growth Factor (CTGF)  ELISA Kit

NSL2158Hu Sunlong 96 wells 468 EUR
Description: Sandwich ELISA

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SEA010Hu-10x96wellstestplate Cloud-Clone 10x96-wells test plate 4578.24 EUR
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

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SEA010Hu-1x48wellstestplate Cloud-Clone 1x48-wells test plate 482.21 EUR
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

SEA010Hu-1x96wellstestplate Cloud-Clone 1x96-wells test plate 637.44 EUR
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

SEA010Hu-5x96wellstestplate Cloud-Clone 5x96-wells test plate 2508.48 EUR
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

4-SEA010Hu Cloud-Clone
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  • 1 plate of 96 wells
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

RD-CTGF-Hu-48T Reddot Biotech 48T 328 EUR
Description: sandwich

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RD-CTGF-Hu-96T Reddot Biotech 96T 469 EUR
Description: sandwich

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

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SL2234Hu Sunlong 96 Tests 468 EUR

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RDR-CTGF-Hu-48T Reddot Biotech 48T 344 EUR
Description: sandwich

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RDR-CTGF-Hu-48Tests Reddot Biotech 48 Tests 469.2 EUR

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RDR-CTGF-Hu-96T Reddot Biotech 96T 492 EUR
Description: sandwich

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

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RK00261 Abclonal 96T 322.37 EUR

Human CTGF/ Connective tissue growth factor ELISA Kit

E0589Hu Sunlong 1 Kit 515 EUR
Description: CCN2,HCS24,IGFBP8,CCN family member 2,Hypertrophic chondrocyte-specific protein 24,Insulin-like growth factor-binding protein 8,IBP-8

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EH0702 FN Test 96T 628.92 EUR
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Description: 37.5 pg/mL

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1-CSB-EP006147HU Cusabio
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  • 10ug
  • 50ug
  • 100ug
  • 200ug
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  • 1MG
Description: Recombinant Human Connective tissue growth factor(CTGF) expressed in E.coli

Human Connective tissue growth factor (CTGF)

1-CSB-EP006147HU1 Cusabio
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  • 10ug
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  • 100ug
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  • 1MG
Description: Recombinant Human Connective tissue growth factor(CTGF),partial expressed in E.coli

ELISA kit for Human Connective tissue growth factor (CTGF)

KTE62960-5platesof96wells Abbkine 5 plates of 96 wells 2702.4 EUR
Description: Quantitative sandwich ELISA for measuring Human Connective tissue growth factor (CTGF) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

20-abx155409 Abbexa
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  • 96 tests
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Rat connective tissue growth factor, CTGF ELISA Kit

CSB-E07876r-24T Cusabio 1 plate of 24 wells 198 EUR
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Rat connective tissue growth factor,CTGF ELISA Kit

CSB-E07876r-48T Cusabio 48T 417.3 EUR
Description: Rattus norvegicus (Rat)

Rat connective tissue growth factor, CTGF ELISA Kit

1-CSB-E07876r Cusabio
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  • 1 plate of 96 wells
  • 5 plates of 96 wells each
  • 10 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat connective tissue growth factor, CTGF in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat connective tissue growth factor,CTGF ELISA Kit

CSB-E07876r-96T Cusabio 96T 642 EUR
Description: Rattus norvegicus (Rat)

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CN-01491R1 ChemNorm 96T 567.6 EUR

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Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

DLR-CTGF-Ra DL Develop 96T 394 EUR
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

DLR-CTGF-Ra-48T DL Develop 48T 544.8 EUR
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

DLR-CTGF-Ra-96T DL Develop 96T 704.4 EUR
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat connective tissue growth factor,CTGF ELISA KIT

E0356Ra-1096T Jiaxing Korain Biotech Ltd (BT Labs) 10*96T 4122 EUR

Rat connective tissue growth factor,CTGF ELISA KIT

E0356Ra-48wells Jiaxing Korain Biotech Ltd (BT Labs) 48 wells 300 EUR

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E0356Ra-596T Jiaxing Korain Biotech Ltd (BT Labs) 5*96T 2061 EUR

Rat connective tissue growth factor,CTGF ELISA KIT

E0356Ra-96wells Jiaxing Korain Biotech Ltd (BT Labs) 96 wells 458 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

DL-CTGF-Ra DL Develop 96T 375 EUR
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat connective tissue growth factor(CTGF)ELISA Kit

GA-E0364RT-48T GenAsia Biotech 48T 380.4 EUR

Rat connective tissue growth factor(CTGF)ELISA Kit

GA-E0364RT-48Tests GenAsia Biotech 48 Tests Ask for price

Rat connective tissue growth factor(CTGF)ELISA Kit

GA-E0364RT-96T GenAsia Biotech 96T 595.2 EUR

Rat connective tissue growth factor(CTGF)ELISA Kit

GA-E0364RT-96Tests GenAsia Biotech 96 Tests 420 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

EKU03438-48T Biomatik Corporation 48T 600.25 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

EKU03438-5x96T Biomatik Corporation 5x96T 4073.13 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

EKU03438-96T Biomatik Corporation 96T 857.5 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

EKU03439-48T Biomatik Corporation 48T 542.78 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

EKU03439-5x96T Biomatik Corporation 5x96T 3683.15 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

EKU03439-96T Biomatik Corporation 96T 775.4 EUR

Rat connective tissue growth factor(CTGF) Elisa Kit

EK720733 AFG Bioscience LLC 96 Wells 0.6 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

EKL54190-5x96T Biomatik Corporation 5x96T 3075.15 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

EKL54190-96T Biomatik Corporation 96T 647.4 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

EKN44549-48T Biomatik Corporation 48T 409.22 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

EKN44549-5x96T Biomatik Corporation 5x96T 2776.85 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

EKN44549-96T Biomatik Corporation 96T 584.6 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

EKN44550-48T Biomatik Corporation 48T 370.02 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

EKN44550-5x96T Biomatik Corporation 5x96T 2510.85 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

EKN44550-96T Biomatik Corporation 96T 528.6 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

EIA05533r Enlibio each 480 EUR

Rat connective tissue growth factor,CTGF ELISA Kit

EKC40339-48T Biomatik Corporation 48T 647.15 EUR

Rat connective tissue growth factor,CTGF ELISA Kit

EKC40339-5x96T Biomatik Corporation 5x96T 4391.38 EUR

Rat connective tissue growth factor,CTGF ELISA Kit

EKC40339-96T Biomatik Corporation 96T 924.5 EUR

Rat connective tissue growth factor,CTGF ELISA KIT

JOT-EK0356Ra Jotbody 96 wells Ask for price
Description: Rat

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS450969-10x96StripWells MyBiosource 10x96-Strip-Wells 4450 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS450969-48StripWells MyBiosource 48-Strip-Wells 390 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS450969-5x96StripWells MyBiosource 5x96-Strip-Wells 2285 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS450969-96StripWells MyBiosource 96-Strip-Wells 515 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS458638-10x96Tests MyBiosource 10x96Tests 4630 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS458638-48Tests MyBiosource 48Tests 405 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS458638-5x96Tests MyBiosource 5x96Tests 2375 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS458638-96Tests MyBiosource 96Tests 535 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS3808051-10x96StripWells MyBiosource 10x96-Strip-Wells 6725 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS3808051-48StripWells MyBiosource 48-Strip-Wells 550 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS3808051-5x96StripWells MyBiosource 5x96-Strip-Wells 3420 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS3808051-96StripWells MyBiosource 96-Strip-Wells 765 EUR

Pig Connective tissue growth factor (CTGF) ELISA Kit

MBS282142-10x96StripWells MyBiosource 10x96-Strip-Wells 6840 EUR

Pig Connective tissue growth factor (CTGF) ELISA Kit

MBS282142-48StripWells MyBiosource 48-Strip-Wells 480 EUR

Pig Connective tissue growth factor (CTGF) ELISA Kit

MBS282142-5x96StripWells MyBiosource 5x96-Strip-Wells 3610 EUR

Pig Connective tissue growth factor (CTGF) ELISA Kit

MBS282142-96StripWells MyBiosource 96-Strip-Wells 805 EUR

Rat connective tissue growth factor, CTGF ELISA Kit

MBS162558-10x96StripWells MyBiosource 10x96-Strip-Wells 3955 EUR

Rat connective tissue growth factor, CTGF ELISA Kit

MBS162558-48StripWells MyBiosource 48-Strip-Wells 305 EUR

Rat connective tissue growth factor, CTGF ELISA Kit

MBS162558-5x96StripWells MyBiosource 5x96-Strip-Wells 2005 EUR

Rat connective tissue growth factor, CTGF ELISA Kit

MBS162558-96StripWells MyBiosource 96-Strip-Wells 475 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2019195-10x96StripWells MyBiosource 10x96-Strip-Wells 5220 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2019195-24StripWells MyBiosource 24-Strip-Wells 355 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2019195-48StripWells MyBiosource 48-Strip-Wells 535 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2019195-5x96StripWells MyBiosource 5x96-Strip-Wells 2860 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2019195-96StripWells MyBiosource 96-Strip-Wells 705 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS261004-10x96StripWells MyBiosource 10x96-Strip-Wells 3290 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS261004-48StripWells MyBiosource 48-Strip-Wells 295 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS261004-5x96StripWells MyBiosource 5x96-Strip-Wells 1800 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS261004-96StripWells MyBiosource 96-Strip-Wells 450 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2700731-10x96StripWells MyBiosource 10x96-Strip-Wells 3370 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2700731-24StripWells MyBiosource 24-Strip-Wells 260 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2700731-48StripWells MyBiosource 48-Strip-Wells 350 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2700731-5x96StripWells MyBiosource 5x96-Strip-Wells 1750 EUR

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2700731-96StripWells MyBiosource 96-Strip-Wells 445 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2700732-10x96StripWells MyBiosource 10x96-Strip-Wells 3060 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2700732-24StripWells MyBiosource 24-Strip-Wells 250 EUR

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

MBS2700732-48StripWells MyBiosource 48-Strip-Wells 325 EUR
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The outcomes of histomorphometric research confirmed that the imply quantity of spermatogonial cells, spermatocytes and spermatids within the experimental group was considerably greater than the management group (with out transplantation) (P < 0.05) and most of the cells responded positively to the detection of DiI.

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  • ANTIBODY
  • blood
  • Camel
  • Chronic exposure to excess iron promotes EMT and cancer via p53 loss in pancreatic cancer.
  • coli recombinant
  • colorimetric
  • colorless
  • Containing
  • Culture
  • DNA
  • ELISA KITS
  • genome
  • GMO
  • Human Cerebrospinal Fluid Induces Neuronal Excitability Changes in Resected Human Neocortical and Hippocampal Brain Slices.
  • Quantitative Imaging of Changes in Astrocytic and Neuronal Adenosine Triphosphate Using Two Different Variants of ATeam.
  • Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells.
  • RNA
  • Sheep
  • Targeting PIK3CG in Combination with Paclitaxel as a Potential Therapeutic Regimen in Claudin-Low Breast Cancer.
  • Cell Culture Media Overview
  • Discover AffiMEDIUM, the New Product Line for Culture Medium
  • Capilia Rapid Antigen
  • Compare antibodies lab reagents for research
  • Compare recombinant lab reagents for research
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